What is sodium deoxycholate used for? Sodium deoxycholate is a bile acid salt. It contains a deoxycholate. A bile acid formed by bacterial action from cholate. It is usually conjugated with glycine or taurine. Deoxycholic acid acts as a detergent to solubilize fats for intestinal absorption, is reabsorbed itself, and is used as a choleretic and detergent.
How do you dissolve deoxycholate?
Sodium deoxycholate precipitates in low pH. Ideally you should dissolve it in a solution buffered at slightly basic pH (~7.4-8). If this condition is not suitable for you always prepare a fresh solution in water and check/adjust the pH accordingly.
How do you make a 10% deoxycholate solution of sodium?
The 10% sodium deoxycholate stock solution (5 g into 50 ml) must be protected from light. The 100 mM EDTA stock solution is made with 1.86 g into 40 ml H2O and then add NaOH to dissolve and adjust pH to 7.4. Finally, adjust the total volume to 50 ml).
How do you dilute sodium deoxycholate?
Add 5 mL of the sodium deoxycholate solution of 10% that is prepared separately in advance (the working concentration of sodium deoxycholate solution is 0.5%), and add 1 mL of NP-40. Mix them and add distilled water until the total volume is 100 mL.
30% Acrylamide.
| Acrylamide | 29 g |
|---|---|
| N,N'-Methylenebisacrylamide | 1 g |
Is sodium deoxycholate toxic?
Ingestion Harmful if swallowed. Harmful if absorbed through skin. May cause skin irritation. Eyes May cause eye irritation.
Related guide for What Is Sodium Deoxycholate Used For?
What does deoxycholate do to cells?
Sodium deoxycholate, the sodium salt of deoxycholic acid, is often used as a biological detergent to lyse cells and solubilise cellular and membrane components.
How do you make a deoxycholate solution?
The 10% sodium deoxycholate stock solution (5 g into 50 ml) must be protected from light. The 100 mM EDTA stock solution is made with 1.86 g into 40 ml H2O and then add NaOH to dissolve and adjust pH to 7.4. Finally, adjust the total volume to 50 ml). Store the buffer at 4°C.
How do you store sodium deoxycholate?
Deoxycholate should be stored at room temperature and must be protected from light. Shelf life is limited and must be used used within a few days (not sure how long exactly but we used to use it all up within 48 hours of reconstitution as it starts precipitating).
Does cholic acid dissolve in water?
Cholic acid, also known as 3α,7α,12α-trihydroxy-5β-cholan-24-oic acid is a primary bile acid that is insoluble in water (soluble in alcohol and acetic acid), it is a white crystalline substance.
How do you make a 50 millimeter Tris base?
How do you make 1M Tris HCl?
For a 1 M solution, dissolve 121.1 g of Tris base in 800 ml of H2O. Adjust the pH to the desired value by adding concentrated HCl. Allow the solution to cool to room temperature before making final adjustments to the pH.
What is Tris HCl used for?
Tris HCL is a buffering agent (acidic buffer) commonly used by molecular biologists to adjust the pH of a solution or stabilize the pH.
Is sodium deoxycholate denatured?
Sodium cholate and sodium deoxycholate are the least denaturing of the commonly used ionic detergents.
Does sodium deoxycholate denature protein?
Sodium deoxycholate and sodium cholate are bile salts detergents. They are both anionic detergents. Deoxycholate does denature proteins while cholate is a non-denaturing detergent.
Is deoxycholic acid the same as deoxycholate?
Deoxycholic acid is also known as deoxycholate and cholanoic acid. Deoxycholic acid emulsifies fats to aid their intestinal absorption. When injected into subcutaneous fat, deoxycholic acid destroys adipocytes (fat cells).
How do you dissolve deoxycholic acid?
Deoxycholic acid (DCA) is supplied as a crystalline solid. A stock solution may be made by dissolving the DCA in the solvent of choice, which should be purged with an inert gas. DCA is soluble in organic solvents such as ethanol, DMSO, and dimethyl formamide (DMF).
What does sodium deoxycholate do in RIPA buffer?
The following RIPA Lysis Buffer components have the following effects: Tris-HCl is a buffering agent prevents protein denaturation, NaCl is a salt that prevents non-specific protein aggregation, NP-40 is a non-ionic detergent to extract proteins; Na-deoxycholate and SDS are ionic detergents to extract proteins; and
Where does sodium deoxycholate come from?
Sodium deoxycholate is a bile acid salt. It contains a deoxycholate. A bile acid formed by bacterial action from cholate. It is usually conjugated with glycine or taurine.
Is sodium deoxycholate a surfactant?
Supramolecular hydrogels were prepared in the mixtures of biological surfactant sodium deoxycholate (NaDC) and halide salts (NaCl and NaBr) in sodium phosphate buffer.
Can you autoclave Tris buffer?
Sterilization of Solutions: Tris solutions can be autoclaved (121°C, 15 psi, 15 minutes) or sterile filtered.
What is the pH of Tris base?
Tris-base 1M is water has pH around 11. Whenever you see Tris in biological protocols, it means Tris base that its pH has been changed to 7-8 and NOT the commercially Tris-Hcl.
What is the pH of Tris HCl?
Tris hydrochloride (Tris Cl) is a buffer used throughout scientific research. Tris Cl is a Good Buffer with an effective pH range between 7.0 and 9.2.
What is RIPA buffer used for?
Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells.
How do you make RIPA buffer?
How to make a RIPA lysis buffer solution. Measure out 3 mL sodium chloride (5 M), 5 mL Tris-HCl (1 M, pH 8.0), 1 mL nonidet P-40, 5 mL sodium deoxycholate (10 %), 1 mL SDS (10%) and add to a 100 mL Duran bottle.
How stable is Digitonin?
Stability of stock solutions: solutions of digitonin may be stored up to one week at +4°C. The capability of digitonin to permeabilize membranes may differ from batch to batch from all suppliers.
Is cholic acid a bile salt?
In humans, taurocholic acid and glycocholic acid (derivatives of cholic acid) and taurochenodeoxycholic acid and glycochenodeoxycholic acid (derivatives of chenodeoxycholic acid) are the major bile salts. They are roughly equal in concentration.
What pH is cholic acid?
Organic solvent-free aqueous solutions of cholic acid can be prepared by directly dissolving the crystalline solid in aqueous buffers. The solubility of cholic acid in PBS, pH 7.2, is approximately 1 mg/ml.
Where does cholic acid come from?
Cholic acid is synthesized from cholesterol in the liver and is conjugated to either glycine (glycocholic acid) or taurine (taurocholic acid) before secretion in the bile. Bile acids act in bile to solubilize cholesterol (which is totally insoluble in water) with phospholipids in mixed micelles.
How do you make 150mM NaCl?
It should always indicate the molecular weight. So, for a 1M solution of NaCl you would dissolve 58.44 grams into 1 liter. There are 1000 mM per M so a 150mM solution is 0.15M. Multiply 58.44 by 0.15 and you have the amount of NaCl you need to make one liter of a 150mM solution.
How do you dilute Tris HCl?
To obtain a 10 mM Tris-HCl pH 7.4 solution, dilute 1 M Tris-HCl pH 7.4 1:100 with nuclease-free water. For example, add 1 mL of 1 M Tris-HCl pH 7.4 to 99 mL of nuclease-free water. Always add an acid to an aqueous solution; never add an aqueous solution to an acid.
How do you make 50 mM EDTA?
How do you make 3m Tris-HCl?
What is trizma hydrochloride?
General description. Trizma® hydrochloride is mainly required for preparation of buffer at physiological range of 7.3 to 7.5. The prepared buffer is compatible with biological fluids. It is of importance to laboratories as a standard pH solution.
How do you make a 1M HCl solution?
Why is tris a buffer?
Biological buffers, like tris, are important because they can maintain a stable pH despite influences that might otherwise shift the pH. Tris(hydroxymethyl) aminomethane, with a pKa of 8.1, is an effective buffer between pH 7 and 9. Because of its neutral range, tris is a commonly used buffer in biological labs.
Does Tris expire?
Shelf life is three years after production date. Tris buffer solutions can be stored at room temperature or at +4°C for two weeks.
What does Tris stand for?
TRIS
| Acronym | Definition |
|---|---|
| TRIS | Tris(Hydroxymethyl)aminomethane |
| TRIS | Tri-State |
| TRIS | Transportation Research Information Service (US Dept. of Transportation) |
| TRIS | Traffic and Road Information System (Canada) |